Journal: iScience

Article Title: Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells

doi: 10.1016/j.isci.2025.114540

Figure Lengend Snippet: Piezo1 activation mediates iron overload under elevated matrix stiffness (A and B) Immunofluorescence images of FerroOrange in endothelial cells on soft and stiff polyacrylamide gels at different time points (0, 3, 6, 12, and 24 h). (Scale bars: 15 μm; along with statistical analysis; n = 3 biological repeats for each group, one-way ANOVA). (C) The intracellular Fe 2+ content was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (D) Calcein-AM quantitative analysis reflects the amount of intracellular labile iron pool (LIP). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (E and F) Immunofluorescence images of FerroOrange staining in endothelial cells from different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) at 0 and 12 h, along with statistical analysis. (Scale bars: 20 μm; n = 3 biological repeats for each group, analyzed by one-way ANOVA). (G) The intracellular Fe 2+ content in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1) was measured using an iron assay kit at different time points. ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). (H) Calcein-AM quantitative analysis reflecting the amount of intracellular labile iron pool (LIP) in different treatment groups (NC, Si-Piezo1, TfR-1-IN-1, and Si-Piezo1+TfR-1-IN-1). ( n = 3 biological repeats for each group, analyzed by one-way ANOVA). Two-sided comparison; all data are mean ± SD; in all bar charts, each dot represents one data point; error bars represent SDs; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns: no significant.

Article Snippet: TfR-1-IN-1 , MCE , Cat#HY-159569.

Techniques: Activation Assay, Immunofluorescence, Iron Assay, Staining, Comparison